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Translational Research Laboratory
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Welcome

Welcome to the University of Southern California FBS Online Portal. This site is designed to automate the use of our Core Facilities and to provide the best possible customer service.

Quick Info

For more info, please contact the Research Initiatives and Infrastructure (RII) Team.

Hours of Operation

Current Time: 7:13 AM

SunOpen All Day
MonOpen All Day
TueOpen All Day
WedOpen All Day
ThuOpen All Day
FriOpen All Day
SatOpen All Day
Our Core Facilities

To learn more about a particular facility or to request access, please click on a facility name below.

Norris Comprehensive Cancer Center

  • Bioreagent and Cell Culture Core
  • Cancer Center Glassware
  • Data Science Core
  • Flow Cytometry and Immune Monitoring
  • Liquid Biopsy Core
  • Molecular and Cell Biology (MCB) Support Core
  • Molecular Genomics / DNA Oligo
  • Molecular Genomics / DNA Sequencing
  • Molecular Genomics Core
  • Molecular Imaging Center – Small Animal Imaging
  • Population Research Core
  • Preclinical Models Shared Resource
  • Translational Pathology Core Facility
  • Translational Pathology Population Based

USC

  • Agilent Center of Excellence
  • Aging Biomarker and Service Core
  • Animal Core
  • Biobehavioral Simulated Environment Research
  • Biosafety Level 3 Research Laboratory
  • Center for Economic and Social Research
  • Center for Image Acquisition
  • Center of Advanced Manufacturing
  • Center of Excellence Molecular Characterization
  • CTR for Liver Diseases–Cell Tissue Imaging Core
  • Department of Animal Resources
  • Diabetes & Obesity Institute's Clinical Core
  • Digital Communication Research Service
  • Dornsife Cognitive Neuroscience Imaging Center
  • Dynamic Imaging Science Center
  • Flow Cytometry Facility
  • Functional Biological Imaging
  • Genome Modification Facility
  • Histology Laboratory
  • John O’Brien Center for Nanofabrication
  • Keck Genomics Platform
  • Machine Shop
  • Medicinal Chemistry Core
  • Metabolic Assay Core for USC Diabetes
  • Multi-Omics Mass Spectrometry Core
  • Multi-Photon Microscopy Core
  • Murine Phenotyping Core
  • Nano Biophysics Core
  • NEI P30 Cell & Tissue Imaging Core
  • NEI P30 Ophthalmic Therapeutics Engineering Core
  • Neuroimaging and Informatics Computing
  • Optical Imaging Facility
  • SC-CTSI - Clinical Trials Unit
  • SC-CTSI Biostatistics, Epidemiology and Research
  • SC-CTSI Clinical Research Informatics
  • SC-CTSI Clinical Research Support
  • Seahorse Core Facility GER
  • Spatial Sciences Institute
  • Structures and Materials Research Lab
  • The Choi Family Therapeutic Screening
  • The Integrative Liver Cell Core
  • Translational Imaging Center
  • Translational Research Laboratory
  • USC Center for Peptide and Protein Engineering
  • USC Core Center of Excellence in Nano Imaging
  • USC Dornsife PLASMA Facility
  • USC Research Initiatives and Infrastructure
  • USC Schaeffer Center Data Core

Main Contact Info

Maryann Vogelsang

1985 Zonal Avenue
USC School of Pharmacy
Los Angeles, CA 90033


trlabcore@usc.edu

(323) 442-0340


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The following Products and Services are available within our facility:

Sample Processing

 

INTEGRA ASSIST PLUS

Electronic multi-channel pipettes (12.5 uL, 300 uL and 1250 uL) along with automated liquid handling robot streamline routine pipetting to ensure superior accuracy, reproducibility without error. Pipetting modes include mixing, dilution, repeat or variable dispense, and multi aspirate for 96- or 384-well plates.

 

KingFisher Duo Prime

Automated purification system for DNA/RNA/protein from a variety of starting materials provides high yield, reproducibility and minimal hand-on time. Auto-decontamination with UV prevents cross-contamination. MagMAX from Thermo Scientific™ or other compatible magnetic beads are applicable.

 

Qiagen TissueLyser II

TissueLyser II simultaneously disrupts multiple biological samples through high-speed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads.

 

ThermoMixer HC

ThermoMixer is for heating, cooling, and mixing almost any of your lab vessels. Improve assay results by dry incubation and mixing of your samples simultaneously.

Analytical Instruments

 

Agilent 4200 TapeStation

Automated microfluidic chip-based electrophoresis analyzer to quantify and qualify DNA/RNA samples (up to 96) for Next Generation Sequencing (NGS), microarray (aCGH) or quantitative PCR (qPCR) workflow

 

Bio-Rad Experion (Bioanalyzer) Automated Electrophoresis System

Microfludic chip-based electrophoresis analysis of DNA, RNA and protein size, quantity and quality.

 

BioTek Epoch2 Microplate Spectrophotometer

High-performance, flexible monochromator-based multi-mode microplate reader for absorbance with new incubator technology (up to 65ºC) with condensation control allows long-term kinetic analysis for cellular and microbial growth. 6- to 384-well plate format, cuvettes and Take3™ Micro-Volume Plate

 

BioTek Synergy H1 Hybrid Multi-mode Plate Reader

High-performance, flexible monochromator-based, hybrid multi-mode microplate reader (200nm - 900nm) for absorbance, fluorescence intensity, time-resolved fluorescence, fluorescence polarization and luminescence-based applications. The new incubator technology (up to 65°C) with condensation control allows long-term kinetic analysis. Additional injectors allow for dynamic assays. 6- to 384-well plate format, cuvettes and Take3™ Micro-Volume Plate.

 

Hidex 300 SL Scintillation Counter

Automatic Liquid Scintillation Counter with 3 PMT detectors enable triple-to-double coincidence ratio (TDCR) counting, which is an absolute counting method for obtaining counting efficiency of the samples without external or internal standard sources. Samples sizes are 40 (20 mL) or 96 (7 mL). Uses MicroWin software to collect, analyze and export data.

 

Hitachi F-2500 Fluorescence Spectrophotometer

Fluorescence monochromator-based analysis (220-730 nm); Wavelength scanning speeds up to 3000 nm/min; 2 nm resolution. Cuvette format

 

Horiba Jobin Yvon Fluorolog-3 Modular Spectrofluorometer

Steady-state and time-resolved characterization of fluorescent samples at 300 - 600nm. Features include single-read, kinetic-read, time-resolved scan, 3D ex/em scan, temperature control.

 

Jasco J-815 Circular dichroism (CD) Spectroscopy

Jasco J-815 Circular dichroism (CD) is dichroism involving circularly polarized light. CD spectroscopy has a wide range of applications: characterization of a protein’s secondary structure (a-helix, ß-sheet), detection of structural changes and stability upon mutagenesis, pH ranges, temperatures, denaturants, buffers, stabilizers, etc. J-815 features peltier temperature control system, standard CD measurement as well as UV/Vis absorbance.

 

NanoDrop One

Quantifies DNA, RNA and protein samples with 1-2 microliter volumes; 190-850 nm spectral range.

Quantifies DNA, RNA and protein samples with 1-2 uL volumes; 190-850 nm spectral range

 

Oxfornd NMR AS400

2-channel superconducting 400 MHz NMR Spectrometer that produces very high-resolution data. Liquid and solid samples are achievable for structural analysis and identification of organic molecules.

 

Perkin Elmer Differential Scanning Calorimetry (DSC) DS-8500

Perkin Elmer Differential Scanning Calorimeter DSC 8500 features thermal analysis technique used to detect transitions like melts, glass transitions, phase changes, and curing; Features double-furnace technology, which directly measures the heat flow difference between two independent furnaces.

 

Qubit 3.0 Fluorometer

Next generation of benchtop fluorometer that accurately measures DNA, RNA and protein

 

Wyatt DynaPro Dynamic Light Scattering (DLS) Plate Reader

Wyatt Dynamic Light Scattering (DLS) DynaPro Plate Reader II measures the hydrodynamic radius of particles, polymers, or proteins in solution in the size range of 2-1000 nm in diameter.

Cell and Immunobilogy Applications

 

Agilent Seahorse XFe96 Analyzer

XFe96 (96-well format) measures mitochondrial respiration and glycolysis to characterize cellular metabolic function in cultures cells and ex-vivo samples.

 

Agilent Seahorse XFp Analyzer

XFp (8-well format) measures mitochondrial respiration and glycolysis to characterize cellular metabolic function in cultures cells and ex-vivo samples.

 

Biosafety Cabinet 531

Approved BUA protocol is required to use.

Approved BUA protocol is required to use.

 

Biosafety Cabinet 612 #1

Approved BUA protocol is required to use.

Approved BUA protocol is required to use.

 

Biosafety Cabinet 612 #2

Approved BUA protocol is required to use.

Approved BUA protocol is required to use.

 

Bio-Rad Bio-Plex Suspension Luminex Array

Bio-Rad Bio-Plex Suspension Array System
Magnetic and polystyrene Luminex bead-based multiplex analysis of up to 100 biomolecules (proteins, peptides or nucleic acids) in a single sample.

 

Bio-Rad ChecmiDoc Touch Gel Imaging System

Gel imaging system that detects colorimetry, densitometry, chemiluminescence and fluorescence.

 

BioTek Epoch 2 Microplate Spectrophotometer

High-performance, flexible monochromator-based multi-mode microplate reader for absorbance with new incubator technology (up to 65ºC) with condensation control allows long-term kinetic analysis for cellular and microbial growth. 6- to 384-well plate format, cuvettes and Take3™ Micro-Volume Plate

 

BioTek Synergy H1 Hybrid Multi-mode Plate Reader

High-performance, flexible monochromator-based, hybrid multi-mode microplate reader (200nm - 900nm) for absorbance, fluorescence intensity, time-resolved fluorescence, fluorescence polarization and luminescence-based applications. The new incubator technology (up to 65°C) with condensation control allows long-term kinetic analysis. Additional injectors allow for dynamic assays. 6- to 384-well plate format, cuvettes and Take3™ Micro-Volume Plate.

 

Countess II FL Automated Cell Counter

Automated cell counter to count cells, monitor fluorescent protein expression, and measure cell viability

 

Meso Scale Discovery (MSD) Sector Imager 2400

MSD features excellent sensitivity and dynamic range for multiplex assay using 96- well plates and reads single-assay, 384-well plates.

 

Shandon Cytospin 4

Provides economical thin-layer preparations from any liquid matrix, especially hypocellular fluids such as spinal fluid and urine

Flow Cytometry

 

BD Aria Fusion

Comprehensive flow cytometry sorter with biosafety regulation provides exceptional multi-color performance and maximal sorting purity and recovery. Furthermore, Aria Fusion is cross-instrumented with our cell analyzers, Fortessa X20 & LSM II. Current 2 lasers are: 1) 488 nm (Em 530, 585, 616, 695, 780 nm and 2) 633 nm (Em 670, 780 nm). Please contact TRL to schedule.

 

BD Fortessa X-20

Flow cytometry analyzer for high-performance multi-color analysis up to 11 fluorochromes with 3 laser: 1) 405 nm (Em 450, 525, 610 nm), 2) 488 nm (Em 530, 575, 610, 695, 780 nm) and 3) 633 nm (Em 670, 730, 780 nm).

 

BD LSRII

Flow Cytometry Analyzer can simultaneously analyze up to 8 colors with 3 lasers: 1) 355 nm (Em 450, 530 nm), 2) 488nm (Em 530, 575, 695, 780 nm), 3) 633nm (Em 660, 780 nm)

Imaging

 

Bio-Rad ChemiDoc Touch Gel Imaging System

Gel imaging system that detects colorimetry, densitometry, chemiluminescence and fluorescence.

 

BioTek Cytation 5 Cell Imaging Multi-Mode Reader

Cytation 5 combines automated, digital high-resolution microscopy and fast, conventional microplate detection in configurable, upgradable platform: 4X, 10X, 20X and 40X magnification in fluorescence (blue, green, red and far-red), brightfield, color-brightfield, and phase contrast, multi-mode detection modules, temperature control and shaker. 6- to 384-well plate, culture dish, flask, or histology slide can be used. Cytation 5 is also applicable with Seahorse for the imaging normalization.

 

iBright FL1000 Gel / Cell Imager

High-performance imaging system for gels, blots and plates can capture images of color-bright field, violet to far-red fluorescence and luminescence. The fluorescence mode can detect up to 4 colors simultaneously at UV to far-red range.

 

Perkin Elmer IVIS Lumina Series III

Imaging of both fluorescent and bioluminescent reporters from green to infrared range. Equipped with an XGI-8 Gas Anesthesia System (users provide their own isoflurane), and can image up to 5 mice at a time.

 

Zeiss Axio Scope A1

Imaging of both fluorescent and bioluminescent reporters. The system is equipped with filter sets 

Fluorescent microscope that scan color bright field, blue, green and red fluorescence images.

 

Zeiss LSM880 + Airyscan Fast Confocal Microscope

Confocal microscope with Airyscan module (fast, super-resolution scan): 9 lasers (355, 405, 458, 488, 514, 561, 594, 633 and 690-1040 nm); up to 32 fluorescence colors; long-term live scan with temperature and CO2 controlled incubator.

Molecular Biology Applications

 

ABI 7900HT Fast Real-Time PCR System

ABI 7900HT Fast Real-Time PCR System
Quantitative Real-Time PCR for 96-well fast and 384-well PCR reaction plate

 

ABI QuantStudio 12K Flex Real-Time PCR System

The QuantStudio 12K Flex OpenArray® Real-Time PCR Instrument and OpenArray® AccuFill™ System combination can process hundreds to thousands of samples per day, enabling mid-density, high-throughput results with ease of use.

 

ABI OpenArray Autofiller

ABI OpenArray Accufill
Accurately loads samples onto OpenArray® Plates

 

Agilent 4200 TapeStation

Automated microfluidic chip-based electrophoresis analyzer to quantify and qualify DNA/RNA samples (up to 96) for Next Generation Sequencing (NGS), microarray (aCGH) or quantitative PCR (qPCR) workflow

 

Bio-Rad Experion (Bioanalyzer) Automated Electrophoresis System

Microfludic chip-based electrophoresis analysis of DNA, RNA and protein size, quantity and quality.

 

Bio-Rad Gene Pulser MXcell (96-well format)

Microplate-based electroporation of mammalian cells - especially useful for primary and hard-to-transfect cell types.

 

Bio-Rad Gene Pulser Xcell (cuvette format)

Cuvette-based electroporation of mammalian, bacterial, and fungal cell types.

 

NanoDrop One

Quantifies DNA, RNA and protein samples with 1-2 uL volumes; 190-850 nm spectral range

 

Qubit 3.0 Fluorometer

Next generation of benchtop fluorometer that accurately measures DNA, RNA and protein

RNA/DNA Sequencing Applications

 

Agilent 4200 TapeStation

Automated microfluidic chip-based electrophoresis analyzer to quantify and qualify DNA/RNA samples (up to 96) for Next Generation Sequencing (NGS), microarray (aCGH) or quantitative PCR (qPCR) workflow

 

BD Rhapsody Express Single-Cell Assay

BD Rhapsody Express and its cartridge enable single-cell capture and molecular indexing of mRNA transcripts to prepare single cell mRNA sequencing libraries. Proprietary BD™ Molecular Indexing technology is used to count individual mRNA molecules.

 

Bio-Rad Experion (Bioanalyzer) Automated Electrophoresis System

Microfludic chip-based electrophoresis analysis of DNA, RNA and protein size, quantity and quality.

 

Illumina NGS MiSeq

Illumina MiSeq can read up to 15 Gb/run, delivering over 600 bases of sequence data per read, allowing a variety of next-generation sequencing applications, including targeted gene, small genome, and amplicon sequencing, 16S metagenomics, and more.

 

NanoDrop One

Quantifies DNA, RNA and protein samples with 1-2 uL volumes; 190-850 nm spectral range

 

Qubit 3.0 Fluorometer

Next generation of benchtop fluorometer that accurately measures DNA, RNA and protein

Citation Reminder


For any publications, presentations, abstracts or other works resulting from the utilization of TRLab or School of Pharmacy resources, we ask that you acknowledge the Translational Research Laboratory. This helps ensure continued support of the lab and lab personnel.

Thanks for your support!
TRLab

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